Comparative Study of Celluloses from Biofilm-Forming Bacteria for Development of Cellulose-Reinforced Products
Mohd Nazarudin Zakaria1, Mohd Fakharulzaman Raja Yahya2, Sukirah Abd Rahman3, Nooramirah Kamaruddin4, Maizatulnisa Othman5, Shahril Anuar Bahari6

1Mohd Nazarudin Zakaria, Department of Applied Sciences, UiTM Shah Alam, Selangor, Malaysia.
2Mohd Fakharulzaman Raja Yahya, Department of Applied Sciences, UiTM Shah Alam, Selangor, Malaysia.
3Sukirah Abd Rahman, Pusat Penyelidikan Bioteknologi dan Nanoteknologi, Ibu Pejabat MARDI, Persiaran MARDI-UPM Serdang, Selangor, Malaysia.
4Nooramirah Kamaruddin, Department of Applied Sciences, UiTM Shah Alam, Selangor, Malaysia.
5Maizatulnisa Othman, Department of Manufacturing and Materials Engineering, Engineering, IIUM, Kuala Lumpur, Malaysia.
6Shahril Anuar Bahari, Department of Applied Sciences, UiTM Shah Alam, Selangor, Malaysia.
Manuscript received on 15 September 2015 | Revised Manuscript received on 20 September 2015 | Manuscript Published on 30 September 2015 | PP: 20-22 | Volume-5 Issue-4, September 2015 | Retrieval Number: D2194095415/15©BEIESP
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Abstract: This study was conducted to compare the celluloses from Acetobacter xylinum and Pseudomonas fluorescens. Results showed that A. xylinum and P. fluorescens produce insoluble and soluble cellulose respectively. The agitation at several speeds was found to affect the form and yield of bacterial celluloses. Based on FTIR spectroscopy, the biochemical composition of A. xylinum cellulose was apparently distinct from that of P. fluorescens with regards to the spectral region between 830 cm-1 and 1400 cm-1 . Sample preparation of P. fluorescens cellulose for XRD analysis was unsuccessful due to its high solubility in culture medium whilst XRD analysis demonstrated the high crystallinity (92.13%) of A. xylinum cellulose. Collectively, the variations between A. xylinum and P. fluorescents celluloses could be observed in terms of cellulose form, cellulose yield, biochemical composition and crystallinity. The findings from this study are expected to assist the industries in choosing the right source of bacterial cellulose for their commercial products.
Keywords: Bacteria Celulose, Acetobacter Xylinum, Pseudomonas Fluorescens, Cellulose Yield, Crystallinity.

Scope of the Article: Production Engineering