Decoding Evolution of Native Fishes in Garhwal Himalaya using Molecular Markers and DNA Barcoding

Madhu Thapliyal1, Bipin Sati2, Ashish Thapliyal3, K. K. Joshi4

1Madhu Thapliyal, Associate Professor and Head, Department of Zoology, Govt. PG College, Maldevta, Raipur, Dehradun, India.

2Bipin Sati, USERC, Govt. of Uttarakhand, Dehradun, India.

3Ashish Thapliyal, Professor, Department of Biotechnology, Graphic Era Deemed to be University, Dehradun, India.

4K. K. Joshi, Department of Environment Science, Graphic Era Hill University, Dehradun, India.

Manuscript received on 13 October 2019 | Revised Manuscript received on 20 October 2019 | Manuscript Published on 29 June 2020 | PP: 9-16 | Volume-8 Issue-10S2 August 2019 | Retrieval Number: J100408810S219/2019©BEIESP | DOI: 10.35940/ijitee.J1004.08810S219

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Abstract: As we are moving forward into the modern era of science, several new technologies have revolutionized various branches of science. Techniques of biodiversity conservation, fish biology etc. has also adapted to modern techniques. For a long time, most of the researches in taxonomy, including fisheries science were based on morphology and traditional methods. After the decade of 90’s, slowly severalmolecular markers like RFLP, RAPD, SNP’s etc. made inroad into taxonomy and fisheries. Molecular markers have several applications in the field of livestock improvement and understanding population dynamics to name a few. Since the 2004, a specific molecular marker, generally known as DNA Barcoding for species identification, came up. This molecular marker is a part of mitochondrial genome that encodes for Cytochrome C Oxidase Unit I (also called as COX or COI). It is advantageous because it has been tested across several animal species and it can differentiate species very well. This marker has also been used as a forensic tool to identify the species. In the current paper, we have used this molecular marker to decode evolution of native fishes of Garhwal Himalayan region. Over 350 barcodes were developed and these barcodes were used to for phylogenetic analysis.

Keywords:  Molecular Markers, DNA Bar-coding, Evolution, Himalaya, Breeding, livestock
Scope of the Article: Social Sciences