Reduction of TNFR2 Expression in Brain Endothelial Cells in Cerebral Malaria Mice Models after Hyperbaric Oxygen Exposure
Prawesty Diah Utami1, Usman Hadi2, Yoes Prijatna Dachlan3, Guritno Suryokusumo4, Loeki Enggar Fitri5

1Dr. Prawesty Diah Utami, Lecturer of Parasitology, Faculty of Medicine, Hang Tuah University, Surabaya, Indonesia.

2Dr. Usman Hadi, Professor, Department of Internal Medicine, Faculty of Medicine, Regional Hospital Dr. Soetomo Hospital, Airlangga University, Surabaya, Indonesia.

3Dr. Yoes Prijatna Dachlan, Professor, Department of Parasitology, Faculty of Medicine, Airlangga University, Surabaya, Indonesia.

4Dr. Guritno Suryokusumo, Professor, Department of Hyperbaric, Faculty of Medicine, Indonesia University, Depok, Indonesia.

5Dr. Loeki Enggar Fitri, Professor, Department of Parasitology, Faculty of Medicine, Brawijaya University, Malang, Indonesia.

Manuscript received on 09 January 2020 | Revised Manuscript received on 05 February 2020 | Manuscript Published on 20 February 2020 | PP: 94-97 | Volume-9 Issue-3S January 2020 | Retrieval Number: C10210193S20/2020©BEIESP | DOI: 10.35940/ijitee.C1021.0193S20

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Abstract: Tumour Necrosis Factor Receptor 2/ TNFR2 is the main receptor of TNF-α expressed by brain endothelial cells and contributes to Nuclear Factor Kappa Beta/ NFκB activation which induces sequestration of leucocyte cells. The role of hyperbaric oxygen/HBO as a regulator of the inflammatory process can be used as a supportive therapeutic approach in cerebral malaria infection. The research aimed to examine the impact of HBO administration on expression of TNFR-2 in brain endothelial cells of mice infected with P. berghei ANKA. P.berghei ANKA / PbA infected C57BL/6 divided into 3 groups with 13 mice per group: G1 (negative control, normal mice); G2 (positive control; mice infected with PbA without HBO); G3 (treatment group; mice infected with PbA with HBO). Observation of TNFR2 expression using immunohistochemical techniques on the last day of treatment. Study results revealed that there has been a significant decrease in TNFR2 expression in brain vascular endothelial cells in the group that received HBO (p <0.05). Exposure to HBO 2.4 ATA for 10 sessions can prevent cerebral malaria by decreasing TNFR2 expression in vascular endothelial cells of the brain of the mice model.

Keywords: Cerebral Malaria, HBO, Plasmodium berghei ANKA, TNFR2.
Scope of the Article: Bio-Science and Bio-Technology