Anti-cancer Effects of Azadirachta indica in Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats
Ricadonna Raissa1, Wibi Riawan2, Anna Safitri3, Ma Asuncion Guiang Beltran4, Aulanni’am Aulanni’am5

1Ricadonna Raissa, Departement of Chemical Science Study Program, Faculty of Mathematics and Natural Science, Universitas Brawijaya, Malang, Indonesia.

2Wibi Riawan, Departement of Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia

3Anna Safitri, Departement of Chemistry, Faculty of Mathematics and Natural Science, Universitas Brawijaya, Malang, Indonesia. 

4Ma Asuncion Guiang Beltran, College of Veterinary Medicine, Tarlac Agricultural University, Camiling, Tarlac, Philippines. 

5Aulanni’am Aulanni’am, Departement of Biochemistry, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia. 

Manuscript received on 11 January 2020 | Revised Manuscript received on 07 February 2020 | Manuscript Published on 20 February 2020 | PP: 304-307 | Volume-9 Issue-3S January 2020 | Retrieval Number: C10710193S20/2020©BEIESP | DOI: 10.35940/ijitee.C1071.0193S20

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Abstract: Hepatocellular carcinoma (HCC) is a primary liver cancer and has a high rate of mortality. Exploration of local herbal as a chemo-preventive drug has been conducted. This present study aimed to observe the anti-cancer effect of hydroethanolic extracts of Azadirachta indica both Indonesia (HEAII) and the Philippines (HEAIP) on diethylnitrosamine (DEN)-induced HCC on Wistar rat model. VEGF/FGF2/CD166/YAP were evaluated using immunohistochemistry. VEGF/FGF2/CD166/YAP were down-regulated in HCC treated with HEAII and HEAIP compare to that non-treatment HCC. Liver function (aminotransferase (AST) and aminotransferase (ALT) activities) was evaluated from serum. Value of AST and ALT were abnormal in the non-treatment HCC and normal in the HCC treated. These results indicated that both extracts were found to possess anti-cancer activity in HCC.

Keywords: Hepatocelullar Carcinoma; Azadirachta indica; Cluster Differential 166 (CD166); Fibroblast Growth Factor 2(FGF-2); yes Associated Protein (YAP); Vascular Endothelial Growth Factor (VEGF).
Scope of the Article: Bio-Science and Bio-Technology